Final products of the reaction are indole, pyruvic acid, ammonium (NH 4 +) and energy. Tryptophanase catalyzes the deamination reaction, during which the amine (-NH 2) group of the tryptophan molecule is removed. Indole is generated by reductive deamination from tryptophan via the intermediate molecule indolepyruvic acid. This division is performed by a chain of a number of different intracellular enzymes, a system generally referred to as "tryptophanase." The indole test is a biochemical test performed on bacterial species to determine the ability of the organism to convert tryptophan into indole.
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